Coding

Part:BBa_K850000:Design

Designed by: Min-Gyu Kim   Group: iGEM12_NYU_Gallatin   (2012-09-15)


AGM1 gene from candida albicans


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1442
    Illegal BglII site found at 1541
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Gibson assembly fragment must have ovelapping sequences of about 50 bp.


Source

The sequence is from the candida genome database orf19.5013 (C. albicans SC5314. It contains a ribosome binding site. It was synthesized in <500bp pieces for Gibson Assembly and assembeled into pSB1C3.

References